Angel Cid-Arregui, MD, PhD

Group Leader

Targeted Tumor Vaccines

German Cancer Research Center (DKFZ)

Im Neuenheimer Feld 280, Heidelberg

Germany


Biography

1976-1982 MD. Complutense University, Madrid.
1985-1988 PhD. European Molecular Biology Laboratory, Heidelberg, and Autonomous University of Madrid. Studies on the activity of the yeast plasma membrane H+-ATPase. Supervisor: Prof. Ramon Serrano.
1989-1993 Postdoc. German Cancer Research Center. Transgenic models for cervical cancer caused by HPV16 and 18. Director: Prof. Harald zur Hausen
1994-1997 European Molecular Biology Laboratory. Studies on transcytosis and neuronal polarity. Group leader: Prof. Carlos Dotti
1997-1999 University of Heidelberg. Use of viral vectors to study neuroepithelial differentiation. Director: Prof. Wieland Huttner
Since 2000 Group leader. German Cancer Research Center. Development of therapeutic vaccines for immunotherapy of cancer. Liposomal targeting of cancer cells.

Research Interest

Scientific Activities

Publications

JOURNALS
Serrano, R., Montesinos, C. and Cid, A. (1986) A temperature-sensitive mutant of the yeast plasma membrane ATPase obtained
by in vitro mutagenesis. FEBS Lett. 208: 143-146
Cid, A., Gancedo, C. and Lagunas, R. (1987) Inactivation of the glucose transport system in sporulating yeast. FEMS Microbiol.
Lett. 41: 59-61
Cid, A., Vara, F. and Serrano, R. (1987) Inhibition of the proton pumping ATPases of yeast and oat root plasma membranes by
dicyclohexylcarbodiimide. Archives Biochem. Biophys. 252: 496-500
Cid, A., Perona, R. and Serrano, R. (1987) Replacement of the promoter of the yeast plasma membrane ATPase gene by a
galactose-dependent promoter and its physiological consequences. Curr. Genet. 12: 105-110
Eraso, P., Cid, A. and Serrano, R. (1988) Tight control of the amount of yeast plasma membrane ATPase during changes in
growth conditions and gene dosage. FEBS Lett. 224: 193-197
Cid, A. and Serrano, R. (1988) Mutations of the yeast plasma membrane H+-ATPase which cause alterd regulation of the
enzyme. J. Biol. Chem. 263: 14134-14139
Cid, A., Auewarakul, P., García-Carrancá A., Ovseiovich, R., Gaissert, H. and Gissmann, L. (1993) Cell-type-specific activity of
the Human Papillomavirus type 18 upstream regulatory region in transgenic mice and its modulation by tetradecanoyl phorbol
acetate and glucocorticoids. J. Virol.. 67: 6742-6752.
Botquin, V., Cid, A. and Schlehofer, J.R. (1993) J. Cancer Res. Clin. Oncol. 119: 24.
Botquin, V., Cid, A. and Schlehofer (1994) Adeno-associated virus type 2 induces differentiation of mouse embryonic cells,
interferes with embryo development and causes early abortion. J. Gen. Virol. 75: 2655-2662.
Auewarakul, P., Gissmann, L. and Cid-Arregui, A. (1994) Targeted expression of the E6 and E7 oncogenes of Human
Papillomavirus type 16 in the epidermis of transgenic mice elicits generalized epidermal hyperplasia involving autocrine factors.
Mol. Cell. Biol. 14: 8250-8258.
Simons, M., Ikonen, E., Tienari, P. J., Cid-Arregui, A., Monning, U., Beyreuther, K. and Dotti, C.G. (1995) Transcytosis of the
amyloid protein precursor in cultured hippocampal neurons. J. Neurosci. Res. 41: 121-128.
Cid-Arregui, A., de Hoop, M. and Dotti, C.G. (1995) Mechanisms of neuronal polarity. Neurobiol. Aging 16: 239-243.
Cid-Arregui, A., Parton, R., Simons, K. and Dotti, C.G. (1995) Nocodazole-dependent and brefeldin A-sensitive processing and
sorting of newly synthesized membrane proteins in cultured neurons. J Neurosci. 15: 4259-4269.
Morales-Peza, N., Auewarakul, P., Juarez, M.V., Garcia- Carranca, A. and Cid-Arregui, A. (2002) Estrogen antagonists repress
the human papillomavirus type 18 early promoter in the genital tract of transgenic mice. Virology, 294:135-140.
Cid-Arregui, A., Juarez, V. and zur Hausen, H. (2003) A synthetic E7 gene of human papillomavirus type 16 that yields
enhanced expression of the protein in mammalian cells and is useful for DNA immunization studies. J Virol. 77:4928-37.
Cid-Arregui, A., Garcia, G., Jacjinovic, M. and zur Hausen, H. (2005) Nuclear import of HPV-16 E7 occurs via an active
mechanism. Eur. J. Cell Biol. 84 (Suppl 55): 45.
Baez-Astua, A., E. Herraez-Hernandez, N. Garbi, H. A. Pasolli, V. Juarez, H. Zur Hausen, and A. Cid-Arregui (2005). Low-dose
adenovirus vaccine encoding chimeric hepatitis B virus surface antigen-human papillomavirus type 16 e7 proteins induces
enhanced e7-specific antibody and cytotoxic T-cell responses J Virol. 79:12807-17.
Villanueva, R., Morales-Peza, N., Castelán-Sánchez, I., García-Villa, E., Cid-Arregui, A., García-Carrancá, A., López-Bayghen,
E. and Gariglio, P. (2006) Heparin (GAG-hed) inhibits LCR activity of Human Papillomavirus type 18 by decreasing AP1 binding.
BMC Cancer 6:218
Darnell, G.A., Schroder, W.S., Antalis, T.M., Lambley, E., Major, L., Gardner, J., Birrell, G., Cid-Arregui, A. and Suhrbier A.
(2007) The accelerated degradation of the retinoblastoma protein mediated by human papillomavirus E7 requires the cysteine
protease calpain. J BIol Chem 282:37492-37500.
Arreygue-Garcia, N, Daneri-Navarro, A, del Toro-Arreola, A, Cid-Arregui, A, Gonzalez-Ramella, O, Jave-Suarez, L, Aguilar-
Lemarroy, A, Troyo-Sanroman, R, Bravo-Cuellar, A, Delgado-Rizo, V, Garcia-Iglesias, T, Hernandez-Flores, G and del toro3
Arreola, S (2008) Augmented serum levels of soluble MICA and reduced NKG2D expression on NK and T cells in patients with
cervical cancer and precursor lesions. BMC Cancer 8:16
Cid-Arregui, A., (2009) Prophylactic HPV Vaccines. The Open Vaccine Journal 2:123-133
Cid-Arregui, A., (2009) Therapeutic Vaccines Against Human Papillomavirus and Cervical Cancer. The Open Virology Journal
3:67-83
Cid-Arregui, A., (2009) Immuntherapie beim Zervixkarzinom: Stand der Dinge. Geburtsh Frauenheilk 2009 Heft 10:957
Cid-Arregui, A., (2009) Neue Strategien in der Immuntherapie von Gebärmutterhalskrebs. Laborwelt 6:22-25
García DA, Cid-Arregui A, Schmitt M, Castillo M, Briceño I, Aristizábal FA (2011) Highly Sensitive Detection and Genotyping of
HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology. Open Virol J. 2011;
5:70-79.
Del Toro-Arreola S, Arreygue-Garcia N, Aguilar-Lemarroy A, Cid-Arregui A, Jimenez-Perez M, Haramati J, Barros-Nuñez P,
Gonzalez-Ramella O, Del Toro-Arreola A, Ortiz-Lazareno P, Hernandez-Flores G, Bravo-Cuellar A, Daneri-Navarro A, Jave-
Suarez LF. (2011) MHC class I-related chain A and B ligands are differentially expressed in human cervical cancer cell
lines.Cancer Cell Int. 2011; 11:15
Juarez, V, Pasolli, H A, Hellwig, A,Garbi, N and Cid Arregui, A. (2012) Virus-Like Particles Harboring CCL19, IL-2 and HPV16 E7
Elicit Protective T Cell Responses in HLA-A2 Transgenic Mice. Open Virol J, Vol. 6, in press
Cid-Arregui, A, Gariglio, P, Kanda, T and Doorbar, J (2012) High-Risk Human Papillomaviruses: Towards a Better Understanding
of the Mechanisms of Viral Transformation, Latency and Immune-Escape. Open Virol J, Vol. 6, in press
Guest editor, special issue "Oncogenic Human Papillomaviruses" in The Open Virology Journal, 2012
Garcia-Chagollan M, Jave-Suarez LF, Haramati J, Sanchez-Hernandez PE, Aguilar-Lemarroy A, Bueno-Topete MR, Pereira-
Suarez AL, Fafutis-Morris M, Cid-Arregui A, del Toro-Arreola S. Substantial increase in the frequency of circulating CD4+NKG2D+
T cells in patients with cervical intraepithelial neoplasia grade 1. J Biomed Sci. 2013 Aug 16;20:60
Cid‐Arregui, A and Juarez, V (2015). Perspectives in the treatment of pancreatic adenocarcinoma. World J Gastroenterol,
volume 31 issue (August 21, 2015)
Cid‐Arregui, A (2016) Book Review: Manual of Childhood Infections. The Open Microbiology Journal 10: 160-161
Garcia DA, Abba MC, Briceño J, Aristizabal FA, Cid Arregui A (2017) DNA methylation pattern in high-grade cervical intraepithelial
neoplasia and cancer revealed by genome-wide methylation analysis of cervical DNA. Integr Mol Med 4(5): 1-13
BOOK CHAPTERS:
de Hoop, M. and Cid-Arregui, A. (1996). Use of Fowl plague, vesicular stomatitis and Semliki forest viruses to study the
mechanisms of neuronal membrane asymmetry. In "Gene transfer into neurons: towards gene therapy of neurological diseases"
(P. R. Lowenstein and L. W. Enquist, eds.). J. Wiley and Sons.
García-Carrancá, A., Santana, C., Guido M. and Cid-Arregui, A. (1998). Analysis of reporter activity in microinjected cells in
culture. In "Microinjection and Transgenesis. Strategies and Protocols. (A. Cid-Arregui and A. García-Carrancá, eds.). Springer-
Verlag.
Morales-Peza, N., García-Carrancá, A., Auewarakul, P. and Cid-Arregui, A. (1998). Transgenic strategies in virus research:
Human Papillomavirus expression and pathogenesis in transgenic mice. In "Microinjection and Transgenesis. Strategies and
Protocols." (A. Cid-Arregui and A. García-Carrancá, eds.). Springer-Verlag, Heidelberg.
Botquin, V., Schlehofer, J. and Cid-Arregui, A. (1998). Mouse preimplantation embryos as an in vivo system to test effects of viral
genes and regulatory sequences in development. In "Microinjection and Transgenesis. Strategies and Protocols." (A. Cid-Arregui
and A. García-Carrancá, eds.). Springer-Verlag, Heidelberg.
Cid-Arregui A. and Lim F. (2000) Construction and Purification of Defective and Amplicon Herpes Simplex Virus Vectors. In Viral
Vectors: Basic Science and Gene Therapy (1999) A. Cid-Arregui. and A. Garcia-Carrancá (eds). BioTechniques Books, Eaton
Publishing Co. Natick, MA.
Oback B., Cid-Arregui A. and Huttner W.B. (2000) Gene Transfer into Mouse Postimplantation Embryos in Culture Using Herpes
Simplex Amplicons. In Viral Vectors: Basic Science and Gene Therapy (1999) A. Cid-Arregui. and A. Garcia-Carrancá (eds).
BioTechniques Books, Eaton Publishing Co. Natick, MA.
Cid-Arregui A. (2000) Use of Herpes Simplex Amplicons for Gene Transfer to Neuroepithelial Cluster Cultures and Embryonic
Brain Slices. In Viral Vectors: Basic Science and Gene Therapy (2000) A. Cid-Arregui. and A. Garcia-Carrancá (eds).
BioTechniques Books, Eaton Publishing Co. Natick, MA.
Cid-Arregui A., Juárez V., Garcia-Iglesias M.J. and García-Carrancá A. (2000) Efficient Expression of Proteins with Recombinant
Alphaviruses. In Viral Vectors: Basic Science and Gene Therapy (1999) A. Cid-Arregui. and A. Garcia-Carrancá (eds).
BioTechniques Books, Eaton Publishing Co. Natick, MA.
4
Cid-Arregui A. and M.V. Juárez (2000) Biosafety Recommendations for Working with Viral Vectors. In Viral Vectors: Basic Science
and Gene Therapy (1999) A. Cid-Arregui. and A. Garcia-Carrancá (eds). BioTechniques Books, Eaton Publishing Co. Natick, MA
Cid-Arregui A. and M.V. Juárez (2007) Vaccines Against HPV and Cervical Cancer. In Cervical Cancer: Research, Diagnostics
And Treatment. A. Aguilar Lemarroy, L. Jave Suarez and R. de Celis (eds). Mexico.
BOOKS EDITED:
Microinjection and Transgenesis. Strategies and Protocols (1998) A. Cid-Arregui. and A. Garcia-Carrancá (eds). Springer Verlag.
Heidelberg.
Viral Vectors: Basic Science and Gene Therapy (2000) A. Cid-Arregui (eds). BioTechniques Books, Eaton Publishing Co. Natick,
MA.

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