Mahmoud Rezk Ali AbouLaila

Associate Professor

Faculty of Veterinary Medicine

Department of Pathology and Parasitology

Damanhour University

Damanhour 22511, Behera, Egypt


Biography

Mahmoud Rezk AbouLaila is an Associate Professor of Parasitology, Faculty of Veterinary Medicine, Damanhour University, Egypt. He was a visiting researcher at Obihiro University of Agriculture and Veterinary Medicine, Japan. He was JSPS fellow at Obihiro University of Agriculture and Veterinary Medicine, Japan. He Obtained his Ph.D. from Obihiro University of Agriculture and Veterinary Medicine, Japan. He worked as an assistant lecturer, lecturer, and Associate professor at the Department of Parasitology, Faculty of Veterinary Medicine, University of Sadat City, Minoufiya, Egypt. During his Ph.D. and the fellowships, he focused on developing diagnostic and drug therapy for Babesia and Theileria Parasites. He also identified the drug target enzymes in Babesia Parasites. He identified some proteins as vaccine candidates for Babesia control. He conducted molecular and serological epidemiological surveys for these parasites worldwide.

Research Interest

Parasite genetics, Molecular biology, Molecular diagnosis, Drug targets, Subunit vaccines

Scientific Activities

Scholarships, Honors, and Awards
1. State Encouragement Award from the Egyptian Academy of Science and Technology 28/5/2017.
2. The First-Class Privilege Medal from the Egyptian President Abdelfattah Al-sisi 6/8/2017.
3. Award of International Publication from the University of Sadat City 2014, 2015, 2016, and 2017.
4. Egyptian Short Postdoctoral Fellowship the Ministry of Higher Education, Egypt November 2014-May,
2015 at Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
5. JSPS postdoctoral fellowship for young researchers from Japan Society for the Promotion of Science,
Japan 2010-2012 at Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
6. Ph.D. scholarship from the Ministry of Higher Education, Egypt to study Ph.D. in Japan, 2005.

Membership in scientific societies
1. Japanese Society of Parasitology.
2. Japanese Society of Veterinary Science.
3. Japanese Society of Tropical Medicine.
4. JSPS Alumni Association in Egypt (JSPSAAE).
5. Egyptian Society of Veterinary Parasitology.

Publications

List of academic publications
1) Book chapter
AbouLaila, M., Igarashi, I. 2013. Green tea and its main chatechin, Epigallocatechin-3-gallate, provide parasitic protection. In Victor R Preedy (ed). Tea in health and disease prevention. Academic Press, London, chapter 105, pp. 1249-1260. (http://www.sciencedirect.com/science/article/pii/B9780123849373001051)
2) Papers published in journals
1. Elsayed, S.M., AbouLaila, M., Rizk, M.A., Elkhatam A., Hilali, M., Abdel-Wahab, A., Yokoyama, N., Igarashi, I. (2018). An epidemiological survey of Theileria equi parasite in horse and donkey in Egypt. Experimental Parasitology. (Under review).
2. Rizk, M.A., El-Sayed, S.A.E.S., AbouLaila, M., Eltayesh, R., Yokoyama, N., Igarashi, I. (2018). Inhibitory effect of ginger on the in vitro and in vivo growth of Babesia and Theileria parasites. Experimental Parasitology. (Under review).
3. AbouLaila, M., Terkawi, M.A., Yokoyama, N., Igarashi, I. (2018). Expression and characterization of the apical membrane antigen-1(AMA-1) homologue from Babesia bigemina. Veterinary Parasitology. (Under review)
4. AbouLaila, M., Rizk, M. A., El-Sayed, S.M., Yokoyama, N., Igarashi, I. (2018). Honey inhibits in vitro growth of four Babesia species and Theileria equi. The Journal of Protozoology Research. (Under review)
5. Rizk, M.A., AbouLaila, M., El-Sayed, S.A.E.S., Yokoyama, N., Igarashi, I. (2018). Inhibitory effects of fluoroquinolone antibiotics on Babesia divergens and Babesia microti, blood parasites of veterinary and zoonotic importance. Infection and Drug Resistance. (Under review)
6. Abdel Aziz, A. R., AbouLaila. M., Aziz, M., Omar, M., Sultan, K., (2018). In vitro and in vivo anthelmintic activity of Pumpkin seeds and Pomegranate peels extracts against Ascaridia galli. Beni-Suef University Journal of Basic and Applied Sciences. (In Press)
7. Rizk, M.A., El-Sayed, S.A.E.S., AbouLaila, M., Eltayesh, R., Yokoyama, N., Igarashi, I. (2017). Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice. Scientific Reports 7 (1), 12774.
8. Touvshintugla, B., AbouLaila, M., Sivakumar, T., Tayebwa, D., Gantuya, S., Naranbaatar, K., Ishiyama, A., Iwatsuki, M., Otoguro, K., Ōmura, S., Terkawi, M., Guswanto, A., Rizk, M., Yokoyama, N., Igarashi, I. (2017). Chemotherapeutic efficacies of a clofazimine and diminazene aceturate combination against piroplasm parasites and their AT-rich DNA-binding activity on Babesia bovis. Scientific Reports 7 (1), 13888.
9. AbouLaila, M., Touvshintugla, B., Rizk, M. A., Elsayed, S.M., Yokoyama, N., Igarashi, I. (2017). Serum-free GIT medium for short-term in vitro cultures of Babesia bigemina, Babesia divergens, and Theileria equi. The Journal of Protozoology Research 27(1-2), 13-22.
10. Rizk, M. A., Elsayed, S.M., AbouLaila, M., Yokoyama, N., Igarashi, I. (2017). Evaluation of the inhibitory effect of N-acetyl-L-cysteine on Babesia and Theileria parasites. Experimental Parasitology 179, 43-48.
11. Abdel Aziz, A. R., Bazh, E. K. A., AbouLaila. M., Sultan, K., (2017). Epidemiological study on the brown dog tick Rhipicephalus sanguineus at Sadat district, Egypt. PSM Veterinary Research 2 (1), 1-5.
12. Guswanto, A., Allamanda, P., Mariamah, E., Munkhjargal, T., Touvshintugla, B., Takemae, H., Sivakumar, T., AbouLaila, M., Terkawi, M.A., Ichikawa, M., Nishikawa, Y., Yokoyama, N., Igarashi, I. (2017). Development of simultaneous immunochromatographic test strip and its application for the serological detection of Babesia bovis and Babesia bigemina in Western Java, Indonesia. Veterinary Parasitology 239, 76-79.
13. Fereig, R.M., Mohamed, S., Mahmoud, H., AbouLaila, M., Guswanto, A., Nguyen, T., Mohamed, A. E. A., Inoue, N., Igarashi, I., Nishikawa, Y. (2017). Seroprevalence of Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginale antibodies among cattle in southern Egypt. Journal of Tick and Tick Borne Diseases 8 (1), 125-131.
14. El-khatam A., AbouLaila, M., Ibrahim M., AbdelGaber M. (2016). Trichomonas gallinae: prevalence and molecular characterization from pigeons in Minoufiya governorate, Egypt. Experimental Parasitology170, 161-167.
15. AbouLaila, M., Yokoyama, N., Igarashi, I. (2016). RNA interference (RNAi) for some genes from Babesia bovis. Research Journal of Applied Biotechnology 2 (1), 81-92.
16. AbouLaila, M., Yokoyama, N., Igarashi, I. (2016). Evaluation of in vitro growth inhibitory effects of ebselen on Babesia and Theileria parasites. Sadat Veterinary Medical Journal 10, 34-38.
17. Fereig, R., Mahmoud, H., Mohamed, S., AbouLaila, M., Abdel-Wahab, A., Osman, S., Zidan, S., El- Khodary, S., Mohamed, A.E.A., Nishikawa, Y. (2016). Seroprevalence and epidemiology of Toxoplasma gondii in farm animals in different regions of Egypt. Veterinary Parasitology: regional studies and reports 3-4, 1-6.
18. Rizk, M. A., Elsayed, S.M., AbouLaila, M., Touvshintugla B., Yokoyama, N., Igarashi, I. (2016). Large-scale drug screening against Babesia divergens parasite using a fluorescence-based high-throughput screening assay. Veterinary Parasitology 227, 93-97.
19. Fereig, R., AbouLaila, M., Mohamed, S., Mahmoud, H., Ali, A. O., Hilali, M., Zaid, A., Mohamed, E. A., Nishikawa, Y. (2016). Serological detection and epidemiology of Neospora caninum and Cryptosporidium parvum antibodies among cattle in southern Egypt. Acta Tropica 162, 206-211.
20. Touvshintugla B., AbouLaila, M., Davaasuren, B., Ishiyama, A., Sivakumar, T., Yokoyama, N., Iwatsuki, M., Otoguro, K., Igarashi, I. (2016). Clofazimine inhibits growth of Babesia and Theileria parasites in vitro and in vivo. Antimicrobial Agents and Chemotherapy 60 (5), 2739-2746.
21. Munkhjargal, T., Aboge, G., Ueno, A., AbouLaila, M., Yokoyama, N., Igarashi, I. (2016). Profilin is a common antigen among bovine and murine Babesia parasites and its immunogenicity and protective properties against human babesiosis caused by Babesia microti. Experimental Parasitology 166, 29-36.
22. Omar, M. A., Salama, A., Elsify, A., Rizk, M. A., Al-Aboody, M. S., AbouLaila. M., Elsayed, S.M., Igarashi, I. (2016). Evaluation of in vitro inhibitory effect of Enoxacin on Babesia and Theileria parasites. Experimental Parasitology 161, 62-67.
23. Abog, E G., Cao, S., Terkawi, M.A., Masatani, T., Goo, Y., Aboulaila, M., Nishikawa, Y., Igarashi, I. Suzuki, H., Xuan, X. (2015). Molecular characterization of Babesia bovis M17 leucine aminopeptidase and inhibition of Babesia growth by bestatin. The Journal of Parasitology 101(5), 536-541.
24. Elbahy, N. M., Elkhatam, A. O., AbouLaila, M., Abdel Aziz, A. R. (2015b). Prevalence of different flatworms infecting ruminants in Menoufia governorate. Menoufia Veterinary Journal 9, 66-77.
25. Elbahy, N. M., Elkhatam, A. O., AbouLaila, M., Abdel Aziz, A. R. (2015a). Microscopical identification and seasonal dynamics of gastrointestinal nematodes in small ruminants at Menoufia province. Menoufia Veterinary Journal 9, 58-65.
26. Elbahy, N. M., Khalafalla, R. E., Elkhatam, A. O., AbouLaila, M. (2014). Evaluation of Eimeria oocyst whole antigen vaccine and the enhancive effect of probiotic on broilers. International Journal of Basic and Applied Sciences 3 (4), 357-362.
27. Zidan, S.A, Amer, S., AbouLaila, M. (2014). Comparison of immunochromatographic test (RIDA®QUICK) and ELISA for detection of Cryptosporidium parvum in children,
immunocompromised patients, and preweaned calves in Egypt. Time Journals of Agriculture and Veterinary Sciences 2(8), 132-138.
28. AbouLaila, M., Davaasuren, B., Salama, A., Ichikawa, M., Terkawi, M. A., Munkhjargal, T., Yokoyama, N., Igarashi, I. (2014b). Evaluation of the inhibitory effects of miltefosine on the growth of Babesia and Theileria parasites. Veterinary Parasitology 204 (3-4), 104-110.
29. AbouLaila, M., Salama, A., Yokoyama, N., Igarashi, I. (2014a). Evaluation of in vitro inhibitory effects of actinonin on Babesia and Theileria equi parasites. Menoufia Veterinary Journal 8 (1), 153-160.
30. Salama, A.A., AbouLaila, M., Terkawi, M.A., Mousa, A., El-Sify, A., Allaam, M., Zaghawa, A., Yokoyama, N., Igarashi, I. (2014). Inhibitory effect of allicin on the growth of Babesia and Theileria equi parasites. Parasitology Research 113 (1), 275-283.
31. Moussa, A., Cao, S., Aboge, G.O., El Kirdasy, A., Terkawi, M. A., Salama, A., Attia, M., AbouLaila, M., Kamyingkird, K., Adjou Moumouni, P. F., Masatani T., Abd El Aziz, S., Moussa, W., Chahan, B., Fukumoto, S., El Ballal, S., Nishikawa, Y., Xuan, X. (2014). Molecular characterization and antigenic properties of a novel Babesia gibsoni glutamic acid-rich protein (BgGARP). Experimental Parasitology 135 (2), 414-420.
32. Terkawi, M. A., Ratthanophart, J., Salama, A. A., AbouLaila, M., Asada, M., Ueno, A., Alhasn, H., Guswanto, A., Masatani, T., Yokoyama, N., Nishikawa, Y., Xuan, X., Igarashi, I. (2013). Molecular characterization of a new Babesia bovis thrombospondin-related anonymous protein (BbTRAP2). PLOS ONE 8 (12), e83305.33. Salama, A.A., Terkawi, M.A., Kawai, S., Aboulaila, M., Nayel, M., Mousa, A., Zaghawa, A., Yokoyama, N., Igarashi, I. (2013). Specific antibody to a conserved region of Babesia apical
membrane antigen-1 inhibited the invasion of B. bovis into the erythrocyte. Experimental Parasitology 135 (3), 623-628.
34. Munkhjargal, T., Sivakumar, T., Battsetseg, B., Nyamjargal, T., AbouLaila, M., Purevtseren, B., Bayarsaikhan, D., Byambaa, B., Terkawi, M.A., Yokoyama, N., Igarashi, I. (2013). Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia. Infection Genetic Evolution 16,178-85.
35. Ueno, A., Terkawi, M.A., Yokoyama, M., Cao, S., Abog,e G., Aboulaila, M., Nishikawa, Y., Xuan, X., Yokoyama, N., Igarashi, I. (2013). Farsenyl pyrophosphate synthase is a potential molecular drug target of risedronate in Babesia bovis. Parasitology International, 62(2), 189-92
36. Salama, A., AbouLaila, M., Moussa, A., Nayel, M., Elsify, A., Terkawi, M. A., Hassan, H., Yokoyama, N., Igarashi, I. (2012). Evaluation of in vitro and in vivo inhibitory effects of fusidic acid on Babesia and Theileria parasites. Veterinary Parasitology 191 (1-2), 1-10.
37. AbouLaila, M., Terkawi, M. A., Seuseu, F. J., Ota, N., Caine, A., Yokoyama, N., Xuan, X., Igarashi, I. (2012). Expression and immunological characterization of heat shock-70 homologue from B. bigemina. Veterinary Parasitology 190 (3-4), 401-410.
38. Munkhjargal, T., AbouLaila, M., Terkawi, M. A., Ueno, A., Sivakumar, T., Yokoyama N., Igarashi I. (2012b). Inhibitory effects of pepstatin A and mefloquine on the growth of Babesia parasites. The American Journal of Tropical Medicine and Hygeine 87 (4), 681-688.
39. Munkhjargal T., AbouLaila M., Sivakumar T., Nakano Y., Yokoyama M., Yokoyama N., Igarashi I. (2012a). Cloning and characterization of histone deacetylase from Babesia bovis. Veterinary Parasitology 190 (3-4), 423-433.
40. Nayel, M., El-Dakhly, K.M., AbouLaila, M., Elsify, A., Hassan, H., Ibrahim, E., Salama, A., Yanai, T. (2012). The use of different diagnostic tools for Babesia and Theileria parasites in cattle in Menofia, Egypt. Parasitology Research 111 (3), 1019-1024.
41. AbouLaila, M., Munkhjargal, T., Sivakumar, T., Ueno, A., Nakano, Y., Yokoyama, M., Yoshinari, T., Nagano, D., Katayama, K., EL-Bahy, N., Yokoyama, N., Igarashi, I. (2012). Apicoplast-targeting antibacterials inhibit the growth of Babesia parasites. Antimicrobial Agents and Chemotherapy 56, 3196-3206.
42. Terkawi, M. A., Alhasn, H., Ueno, A., Katthanaphart, J., Luo, Y., Cao, S., Kamyingkird, K., Aboulaila, M., Goo, Y., Nishikawa, Y., Xuan, X., Yokoyama, N., Igarashi, I. (2012). The C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses. Parasitology International 61, 493-496.
43. Sivakumar, T., Khukhuu, A., Batesteg, B., Battor, B., AbouLaila, M., Munkhjargal, T., Yoshinari, T., Yokoyama, N., Igarashi, I. (2012). Genetic detection of Babesia bigemina from Mongolian cattle using apical membrane antigen-1 gene based PCR technique. Veterinary Parasitology 187, 17-22.
44. AbouLaila, M., El-Bahy, N., Hilali, M., Yokoyama, N., Igarashi, I. (2011c). Serodiagnosis of Toxoplasma gondii in ducks from Behera Governorate, Egypt. The Journal of Protozoology Research 21, 45-49.
45. AbouLaila, M., El-Bahy, N., Hilali, M., Yokoyama, N., Igarashi, M. (2011b). Prevalence of the enteric parasites of ducks from Behera governorate, Egypt. The Journal of Protozoology Research 21, 36-44.
46. AbouLaila, M., Terkawi, M.A., Yokoyama, N., Igarashi, I. (2011a). In vitro growth inhibitory effect of (-)-Epigallocatechin-3-gallate from green tea on the growth of equine Babesia parasites. The Journal of Protozoology Research 21, 30-35.
47. Terkawi M. A., Seuseu, F.J., Wibowo, P.E., Huyen, N. X., Minoda, Y., AbouLaila, M., Kawai, S., Yokoyama, N., Xuan, X., Igarashi, I. (2011c). Secretion of a new Spherical body protein of Babesia bovis into the cytoplasm of infected erythrocytes. Molecular and Biochemical Parasitology 178 (1-2), 40-45.
48. Terkawi, M. A., Huyen, N. X., Shinuo C. Inpankaew T., Maklon, K., AbouLaila, M., Ueno, A., Y. Goo, Yokoyama, N., Jittapalapong, S., Xuan, X., Igarashi, I. (2011b). Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the northeast region of Thailand. Veterinary Parasitology 178 (3-4), 201-207.
49. Terkawi, M. A., Huyen, N. X., Wibowo, P. E., Seuseu, F. J., AbouLaila, M., Ueno, A., Goo, Y., Yokoyama, N., Xuan, X. , Igarashi, I. (2011a). Spherical body protein 4 is a new serological antigen for the global detection of Babesia bovis infection in cattle. Clinical and Vaccine Immunology 18, 337-342.
50. AbouLaila, M., Yokoyama N., Igarashi, I. (2010e). Development and evaluation of two nested PCR assays for the diagnosis of Babesia bovis from cattle blood. Veterinary Parasitology 172(1-2), 27, 65-70.
51. AbouLaila, M., Sivakumar, T., Yokoyama N., Igarashi, I. (2010d). Inhibitory effect of terpene nerolidol on the growth of Babesia parasites. Parasitology International 59 (2), 278-282.
52. Aboulaila, M., Yokoyama, N., Igarashi, I. (2010c). Development and evaluation of a nested PCR based on spherical body protein 2 gene for the diagnosis of Babesia bovis infection. Veterinary Parasitology 169, 45-50.
53. AbouLaila, M., Nakamura, K., Govind, Y. Yokoyama N., Igarashi, I. (2010a). Evaluation of the in vitro growth-inhibitory effect of epoxomicin on Babesia parasites. Veterinary Parasitology 167, 19-27.
54. AbouLaila, M., Yokoyama N., Igarashi, I. (2010b). Inhibitory effects of (-)-Epigallocatechin-3-gallate from green tea on the growth of Babesia parasites.
Parasitology 137 (5), 785-791.
55. Sivakumar, T., Khukhuu, A., Alhassan, A., AbouLaila, M., Iseki, H., Ota, N., Lijia, Z., Yokoyama, N., Igarashi, I. (2009). Expression analysis of a Babesia bovis BboP67 gene
homologous to the Theileria parva p67 gene. The Journal of Protozoology Research 19, 62-71.
56. Munkhjargal, T., AbouLaila, M., Sivakumar, T., Yokoyama, N., Igarashi, I. (2009). Inhibitory effect of apicidin on in vitro and in vivo growth of Babesia parasites. The Journal of Protozoology Research 19, 42-49.
57. Sivakumar, T., AbouLaila, M., Khukhuu, A., Iseki, H., Alhassan, A., Yokoyama, N., Igarashi, I. (2008). In vitro inhibitory effect of fosmidomycin on the asexual growth of
Babesia bovis and Babesia bigemina. The Journal of Protozoology Research 18, 71-78.

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