Mohamed Abdo Rizk, PhD

Assistant Professor

Department of Internal Medicine and Infectious Diseases

Faculty of Veterinary Medicine

Mansoura University, Mansoura 35516, Egypt


Biography

Dr. Mohamed Abdo Rizk holds a DVM degree from Faculty of Veterinary Medicine, Mansoura University. He obtained his Master degree in 2011 in veterinary internal medicine from School of Veterinary Medicine, Mansoura University and obtained his joint PhD (Egypt/Japan) at 2016 in internal medicine from Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan. Dr. Mohamed Abdo Rizk has more than 8 years of experience in veterinary internal medicine and infectious diseases. Beside his research experience in that area, he has 9 years of teaching experience primarily related to teaching veterinary internal medicine and infectious diseases to DVM students. He has many publications in national and international scientific journals. He received the prize of the best PhD thesis in Mansoura University in 2016. He trained in horse clinic and small clinic in University of Veterinary Medicine Hannover, Hannover, Germany. He worked as a foreign visiting researcher at National Research Center of Protozoan Diseases (NRCPD), Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan. Currently, he is an assistant professor of internal medicine and infectious diseases at Faculty of Veterinary Medicine, Mansoura University.

Research Interest

Dr. Mohamed Abdo Rizk main area of interest is treatment and diagnosis of protozoan diseases especially Babesia and Theileria parasites as my PhD was on treatment and diagnosis of Babesia parasite. Besides treatment and diagnosis of respiratory diseases in calves, and animal infectious diseases.

Main Past Research Achievements and Results
1) Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice: The major objective of this study is the development of high-throughput screening assay for evaluation the drugs against B. microti infection in mice, instead of determination of parasitemia by microscopy method that requires preparation of Giemsa-stained thin blood smears then counting RBCs for each slide which is a time-consuming procedure. The results revealed the good agreement between the emitted fluorescence signals obtained by fluorescence assay and the parasitemia obtained by microscopy method at 2.5% HCT using diminazene aceturate that was used as already commercial available drug. Considering that all experiments were applied under optimal laboratory conditions, fluorescence assay at 2.5% HCT using 2x SG I for B. microti parasite offers a novel approach for drug screening in mice. Scientific Reports (In press). This study was supported by a medicine for malaria venture MMV grunt.
2) Evaluation of a Fluorescence-Based Method for Antibabesial Drug Screening: Although microscopy is widely used to calculate parasitemia, several disadvantages are associated with this technique. In this study we evaluated for first time a fluorescence-based method using SYBR green I stain (SG I) to screen antibabesial agents in in vitro cultures of Babesia bovis. The obtained findings suggested that the fluorescence-based method might be useful for antibabesial drug screening and may have potential to be developed into a high-throughput screening (HTS) assay. (Antimicrob Agents Chemother. 58 (2014) 4713-7).
3) Optimization of a Fluorescence-Based Assay for Large-Scale Drug Screening against Babesia and Theileria Parasites: Though, we establishment a fluorescence-based assay to screen antibabesial agents in in vitro cultures of Babesia bovis (Antimicrob Agents Chemother. 58 (2014) 4713-7), the assay wasn’t valid for a large scale antibabesial drug screening and wasn’t established for other Babesia and Theileria parasites. Therefore, we optimized the assay in this study for large-scale drug screening using three different hematocrits (HCTs; 2.5%, 5%, and 10%) without daily replacement of the medium. The results revealed that fluorescence-based assay uses low HCT and does not require daily replacement of culture medium, making it highly suitable for in vitro large-scale drug screening against Babesia and Theileria parasites that infect cattle and horses. (PLOS ONE 10 (2015) 1-15).
4) Cocktail of Theileria equi antigens for detecting infection in equines
: In this study, a cocktail-ELISA containing two antigens (equi merozoite antigen-2 (EMA-2) + Theileria equi (T. equi) protein 82 (Te 82)) to diagnose T. equi infection either in experimentally infected horses or in field infection was applied. Our findings have revealed that a cocktail formula of EMA-2 + Te 82 provided a more practical and sensitive diagnostic candidate for diagnosing T. equi infection in horses as compared with Te 82 or Te 43 alone. (Asian Pac J Trop Biomed.12 (2015) 977–981).
5) An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt: In this study, we analyzed the infection rates of Babesia bovis, Babesia bigemina, Theileria annulata, and Theileria orientalis, using parasite-specific PCR assays in blood– DNA samples sourced from cattle (n = 439), buffaloes (n = 50), and sheep (n = 105) reared in Menoufia, Behera, Giza, and Sohag provinces of Egypt. T. orientalis was firstly reported in Egypt in this study, suggests that T. orientalis infection may have veterinary and economic significance in Egypt. (Parasitol Int. 64 (2015):79-85).
6) Large-scale drug screening against Babesia divergens parasite using a fluorescence-based high-throughput screening assay: The validation of a fluorescence-based high-throughput screening (HTS) assay for determining the efficacies of large chemical libraries against Babesia divergens (bovine strain) in in vitro cultures was evaluated in this study using seven different drugs, including diminazene aceturate, tetracycline hydrochloride, chloroquine diphosphate, pyronaridine tetraphosphate, luteolin, actinonin, and (−)-epigallocatechin-3-gallate from green tea. The data indicated that 5% HCT without daily replacement of the culture medium mixed with bovine serum in vitro using a fluorescence-based HTS assay creates the best conditions for large-scale drug screening against B. divergens that infect cattle. (Vet Parasit., 277(2016); 93-97).
7) Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond: In this study we reported for the first time on 236 screens that have been carried out against the Malaria Box .The combined biochemical and cellular assays presented in this study suggested mechanisms of action for 135 (34%) of the compounds active in killing multiple life-cycle stages of the malaria parasite, including asexual blood, liver, gametocyte, gametes and insect ookinete stages. In addition, many compounds demonstrated activity against other pathogens, showing hits in assays with 16 protozoa, 7 helminthes, 9 bacterial and mycobacterial species, the dengue fever mosquito vector, and the NCI60 human cancer cell line panel of 60 human tumor cell lines. These results reveal the immense potential for translating the dispersed expertise in biological assays involving human pathogens into drug discovery starting points, by providing open access to new families of molecules, and emphasize how a small additional investment made to help acquire and distribute compounds, and sharing the data, can catalyze drug discovery for dozens of different indications. (PLoS Pathogens 12 (2016): e1005763.).This study was supported by a medicine for malaria venture MMV grunt.
8) Evaluation of in vitro inhibitory effect of enoxacin on Babesia and Theileria parasites: In this study, enoxacin was tested as a potent inhibitor against the in vitro growth of bovine and equine Piroplasms. Enoxacin IC50 values for Babesia and Theileria parasites were satisfactory as the drug is potent antibacterial drug with minimum side effects. Therefore, enoxacin might be used for treatment of Babesiosis and Theileriosis especially in case of mixed infections with bacterial diseases or incase of animal sensitivity against diminazene toxicity. (Exp Parasitol.161(2016):62-67).
9) Evaluation of the inhibitory effect of N-acetyl-L-cysteine on Babesia and Theileria parasites. The in vitro inhibitory effect of N-acetyl-L-cysteine was evaluated in the present study on the growth of Babesia and Theileria parasites. The in vitro growth of Babesia bovis, Babesia bigemina, Babesia divergens, Theileria equi, and Babesia caballi that were tested was significantly inhibited (P < 0.05) by micromolar concentrations of N-acetyl-L-cysteine. The inhibitory effect of N-acetyl-L-cysteine was synergistically potentiated when used in combination with diminazene aceturate on B. bovis and B. caballi cultures. These results indicate that N-acetyl-L-cysteine might be used as a drug for the treatment of babesiosis, especially when used in combination with diminazene aceturate.(Exp Parasitol. 179 (2017) 43-48). >
10) Molecular identification and antigenic characterization of Babesia divergens Erythrocyte Binding Protein (BdEBP) as a potential vaccine candidate. In this study, recombinant protein encoding Babesia divergens Erythrocyte Binding Protein (BdEBP) was produced in Escherichia coli, and its antiserum was prepared in mice for further molecular characterization. Western blotting and indirect fluorescent antibody test (IFAT) revealed the specific reaction of the anti-rBdEBP serum with a corresponding authentic protein of B. divergens. Next, bovine RBCs were incubated with a B. divergens lysate, and anti-rBdEBP serum was produced in mice to detect the ability of BdEBP to bind with host cells. The efficacy of pre-incubated free merozoites to invade bovine erythrocytes was inhibited by 60% after incubation with 2 mg/ml of anti-rBdEBP serum for 6 h. The obtained data suggest the possible use of rBdEBP as a vaccine candidate against bovine babesiosis. Parasitology International 66 (2017) 721-726.
11) Animal level risk factors associated with Babesia and Theileria infections in cattle in Egypt. In this study, risk factors and logistic regression statistical analysis was carried out to appraise the potential factors on the cows infected by hemoparasites at three main regions of Egypt (northern, central and southern). The results of the present study declare the prevalent bovine Babesia and Theileria sp. in Egypt based on molecular description. An impression on the potential risk factors associated with infections was obtained. Recognition of the potential risk factors associated with tick borne dis- ease may be helpful to construct the best preventive measures. Acta Parasitol. In press, (2017)
. DOI: 10.1515/ap-2017-00.
12) Comparative therapeutic effect of steroidal and non- steroidal anti-inflammatory drugs on proinflammatory cytokines production in water buffalo calves (Bubalus bubalis) naturally infected with bronchopneumonia: a randomized clinical trial. In the current study, we compared the therapeutic effects of a non-steroidal and a steroidal anti-inflammatory drug on the production of pro-inflammatory cytokines, interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-12p40 (IL-12p40), interferon gamma (IFNγ), and tumor necrosis factor alpha (TNF-α) in the blood of water buffalo (Bubalus bubalis) calves naturally infected by bronchopneumonia. The obtained results indicate the selectively potent anti-inflammatory effect of flunixin meglumine on the production of pro-inflammatory cytokines in pneumonic buffalo calves and highlight the efficacy of flunixin meglumine in the treatment of bronchopneumonia in buffalo calves when used in combination with tulathromycin. Trop Anim Health Prod. In press, (2017)
. DOI 10.1007/s11250-017-1383-8
Current Research
Currently I applied mass screening of malaria box hits on B. microti infected mice. The results obtained from this research will help in fulfilling the empty antibabesial pipeline. This study is within aproject supported by a medicine for malaria venture MMV grunt.

Scientific Activities

Professional fellowships and grants of research
I. Fellowship from Japan Society for the Promotion of Science (JSPS) for Postdoctoral Research (Standard program). (2018-2020)
II. Fellowship from the cultural affairs and missions sector, Egypt to pursue doctoral degree (PhD) as channel mission at Obihiro University of Agriculture and Veterinary Medicine. (2013- 2015).

Memberships in Scientific Society
I. Member of Japanese Society of Veterinary Science
II. Member of Japanese Society of Tropical Medicine
III. Member of Japanese Society of Parasitology

Scientific Awards
The best PhD thesis in Mansoura University in 2016

Publications

List of publications in Refereed International Journals
1) Rizk, M.A., AbouLaila, M., El-Sayed, S., Guswanto A., Yokoyama, N., & Igarashi, I. Inhibitory effects of fluoroquinolone antibiotics on Babesia divergens and Babesia microti, blood parasites of veterinary and zoonotic importance. Infection and Drug Resistance. (2018) In press. (Impact factor 3.77).
2) Hamed M., Rizk M.A., Hafez A., El-Mahdi A.S., El-khodery S. A. Comparative effect of intravenous administration of medetomidine, tramadol and medetomidine/tramadol combination on intraocular pressure (IOP) in clinically healthy donkeys (Equus a sinus). Journal of equine veterinary science. (2018) DOI: 10.1016/J.Jevs.2018.06.001 (Impact factor 0.8)
3) Tayebwa D.S., Tuvshintulga B., Guswanto A., Nugraha A.B., Batiha G.E., Gantuya S., Rizk M.A., Vudriko P., Sivakumar T., Yokoyama N., Igarashi I. The effects of nitidine chloride and camptothecin on the growth of Babesia and Theileria parasites. Ticks and Tick- borne
Diseases. (2018) DOI: 110.1016 (Impact factor 3.23).
4) El-Sayed, S.A.E.S., Rizk, M.A.¶, *. Significance of selected biochemical markers in predicting the outcome of schistosomiasis, J HELLENIC VET MED SOC., (2018); 69 (1), 739-748 (Impact factor 0. 326).
5) AbouLaila, M., Rizk, M.A., El-Sayed, S., Yokoyama, N., & Igarashi, I. In vitro antiparasitic effects of six beverages on the growth of Babesia and Theileria parasites. Annals of Complementary and Alternative Medicine (2018); 3 (1); 3-8.
6) Guswanto A., Nugraha A.B., Tuvshintulga B., Tayebwa D.S., Rizk M.A., Batiha G.E., Gantuya S., Sivakumar T., Yokoyama N., Igarashi I. 17-DMAG inhibits the multiplication of several Babesia species and Theileria equi on in vitro cultures, and Babesia microti in mice. IJP: Drugs and Drug Resistance 8 (2018) 104–111. (Impact factor 4.80).
7) Ali, M.O., El-Adl, M.A., Ibrahim, H.M.M., ElSeedy, Y.Y., Rizk, M.A., El-Khodery S. Molecular characterization f the vitamin D receptor (VDR) gene in Holstein cows. Research in Veterinary Science (2018), 118; 146-150. (Impact factor 1.29).
8) Rizk, M.A., Osman S.A., El-Gaabary M.H., El-Khodery S. Comparative clinical and parasitological efficacy of moxidectin pour on, ivermectin and piprazine citrate on Toxocara vitulorum infection in buffalo calves (Bubalus bubalis): Arandomized clinical trial. Turkish
journal of Veterinary and Animal Sciences (2018) DOI: 10.3906/vet-1707-42
9) Rizk, M.A., El-Sayed, S.A.E.S., AbouLaila, M., Eltayesh R.,Yokoyama, N., & Igarashi, I. Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice. Scientific Reports. Scientific reports (2017) 7: 12774
DOI:10.1038/s41598-017-13052-5 (Impact factor 5.04).
10) Tuvshintulga B., AbouLaila M., Sivakumar T., Tayebwa, D.S., Gantuya S., Naranbaatar K., Ishiyama A., Iwatsuki M., Otoguro K., Ōmura S., Terkawi M.A., Guswanto A., Rizk M.A., Yokoyama N., Igarashi I. Chemotherapeutic efficacies of clofazimine and diminazene aceturate combination against piroplasm parasites as AT-rich DNA-binding drugs. Scientific reports (2017) 7: 13888 | DOI:10.1038/s41598-017-14304-0 (Impact factor 5.04).
11) Rizk, M.A.*, Abdalla, A.A., El-Sayed, S.A.E.S. Evaluation of Ascorbic Acid in Combination of Ivermectin in Augmentation the Recovery from Juvenile Generalized Demodicosis in Dogs: A Randomized Clinical Trial. PSM Vet. Res., 2(2): 14-21 (2017).
12) Rizk, M.A., Salama A., El-Sayed, S.A.E.S., Elsify A., El-ashkar M., Ibrahim H., Youssef M., El-Khodery S. Animal level risk factors associated with Babesia and Theileria infections in cattle in Egypt. Acta Parasitol. 62: 4 (2017). (Impact factor 1.29).
13) Rizk, M.A. *, Mahmoud M.E., El-Sayed, S.A.E.S., Salman D. Comparative therapeutic effect of steroidal and non- steroidal anti-inflammatory drugs on proinflammatory cytokines production in water buffalo calves (Bubalus bubalis) naturally infected with
bronchopneumonia: a randomized clinical trial. Trop Anim Health Prod. 49:1723–1731 (2017). (Impact factor 0.912).
14) AbouLaila, M., Tuvshintulga, B., Rizk, M.A., El-Sayed, S.A.E.S., Yokoyama, N., & Igarashi, I. Serum-free GIT medium for short-term in vitro cultures of Babesia bigemina, Babesia divergens, and Theileria equi . J. Protozool. Res. 27. 13-22 (2017)
15) El-Sayed, S.A.E.S., Rizk, M.A.¶, Terkawi, M.A., Yokoyama, N., & Igarashi, I. Molecular identification and antigenic characterization of Babesia divergens Erythrocyte Binding Protein (BdEBP) as a potential vaccine candidate. Parasitol Int. 66, 721-726 (2017). (Impact factor 1.744).
16) Rizk, M.A., El-Sayed, S.A.E.S., AbouLaila, M., Yokoyama, N., & Igarashi, I. Evaluation of the inhibitory effect of N-acetyl-L-cysteine on Babesia and Theileria parasites. Exp Parasitol.179, 43-48, June, (2017). (Impact factor 1.724).
17) Rizk, M.A.*, Ahmed, A., El-Sayed, S.A.E.S. A comparative study on selected APP, alkaline phosphatase and lactate dehydrogenase activities in buffalo and cow with subclinical mastitis. Comp Clin Pathol., 26, 599-603, February, (2017). (Impact factor 0.358).
18) Rizk, M.A., El-Sayed, S.A.E.S., AbouLaila, M., Tuvshintulga, B., Yokoyama, N., & Igarashi, I. Large-scale drug screening against Babesia divergens parasite using a fluorescence-based high-throughput screening assay. Vet Parasit., 277, 93-97, August, (2016). (Impact factor 2.356).
19) Van Voorhis WC, Adams JH, Adelfio R, Ahyong V, Akabas MH, Alano P, Alday A, Alemán Resto Y, Alsibaee A, Alzualde A, Andrews KT, Avery SV, Avery VM, Ayong L, Baker M, Baker S, Ben Mamoun C, Bhatia S, Bickle Q, Bounaadja L, Bowling T, Bosch J, Boucher
LE, Boyom FF, Brea J, Brennan M, Burton A, Caffrey CR, Camarda G, Carrasquilla M, Carter D, Belen Cassera M, Chih-Chien Cheng K, Chindaudomsate W, Chubb A, Colon BL, Colón- López DD, Corbett Y, Crowther GJ, Cowan N, D'Alessandro S, Le Dang N, Delves M, DeRisi JL, Du AY, Duffy S, Abd El-Salam El-Sayed S, Ferdig MT, Fernández Robledo JA, Fidock DA, Florent I, Fokou PV, Galstian A, Gamo FJ, Gokool S, Gold B, Golub T, Goldgof GM, Guha R, Guiguemde WA, Gural N, Guy RK, Hansen MA, Hanson KK, Hemphill A, Hooft van Huijsduijnen R, Horii T, Horrocks P, Hughes TB, Huston C, Igarashi I, Ingram- Sieber K, Itoe MA, Jadhav A, Naranuntarat Jensen A, Jensen LT, Jiang RH, Kaiser A, Keiser J, Ketas T, Kicka S, Kim S, Kirk K, Kumar VP, Kyle DE, Lafuente MJ, Landfear S, Lee N, Lee S, Lehane AM, Li F, Little D, Liu L, Llinás M, Loza MI, Lubar A, Lucantoni L, Lucet I, Maes L, Mancama D, Mansour NR, March S, McGowan S, Medina Vera I, Meister S, Mercer L, Mestres J, Mfopa AN, Misra RN, Moon S, Moore JP, Morais Rodrigues da Costa
F, Müller J, Muriana A, Nakazawa Hewitt S, Nare B, Nathan C, Narraidoo N, Nawaratna S, Ojo KK1, Ortiz D, Panic G, Papadatos G, Parapini S, Patra K, Pham N, Prats S, Plouffe DM, Poulsen SA, Pradhan A, Quevedo C, Quinn RJ, Rice CA, Rizk M.A., Ruecker A, St
Onge R, Salgado Ferreira R, Samra J, Robinett NG, Schlecht U, Schmitt M, Silva Villela F, Silvestrini F, Sinden R, Smith DA, Soldati T, Spitzmüller A, Stamm SM, Sullivan DJ, Sullivan W, Suresh S, Suzuki BM, Suzuki Y, Swamidass SJ, Taramelli D, Tchokouaha LR, Theron A, Thomas D, Tonissen KF, Townson S, Tripathi AK, Trofimov V, Udenze KO, Ullah I, Vallieres C, Vigil E, Vinetz JM, Voong Vinh P, Vu H, Watanabe NA, Weatherby K, White PM, Wilks AF, Winzeler EA, Wojcik E, Wree M, Wu W, Yokoyama N, Zollo PH, Abla N, Blasco B, Burrows J, Laleu B, Leroy D, Spangenberg T, Wells T, Willis PA. Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond. PLoS Pathogens 12, July: e1005763, (2016). (Impact factor 7.64).
20) Omar, M.A., Salama, A., Elsify, A., Rizk, M.A., Al-Aboody, M.S., AbouLaila, M., El-Sayed, S.A.E.S., Igarashi, I., Evaluation of in vitro inhibitory effect of enoxacin on Babesia and Theileria parasites, Exp Parasitol.,161:62-7, February (2016). (Impact factor 1.724).
21) El-Sayed, S.A., Rizk, M.A. ¶, Terkawi, M.A., Mousa, A., El Said, E.S.E.S., Elsayed, G., Fouda, M., Yokoyama, N., Igarashi, I., Cocktail of Theileria equi antigens for detecting infection in equines, Asian Pac J Trop Biomed., 12: 977–981, May, (2015).
22) Rizk, M.A., El-Sayed, S.A., Terkawi, M.A., Youssef, M.A., El Said el Sel, S., Elsayed, G., El- Khodery, S., El-Ashker, M., Elsify, A., Omar, M., Salama, A., Yokoyama, N., Igarashi, I., Optimization of a Fluorescence-Based Assay for Large-Scale Drug Screening against Babesia
and Theileria Parasites, PLoS ONE, 10: 1-15, April, (2015). (Impact factor 3.057).
23) Elsify, A., Sivakumar, T., Nayel, M., Salama, A., Elkhtam, A., Rizk, M., Mosaab, O., Sultan, K., Elsayed, S., Igarashi, I. and Yokoyama, N., An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt, Parasitol Int. 64:79-85,
February, (2015). (Impact factor 1.744).
24) Youssef, M.A., El-khodery, S.A., Abdo M., A comparative study on selected acute-phase proteins (APPs) and immunoglobulins in buffalo and bovine calves with respiratory disease, Comp Clin Pathol, 24:515–520, (2015). (Impact factor 0.358).
25) Guswanto, A., Sivakumar, T., Rizk, M.A., Elsayed, S.A.E., Youssef, M.A., ElSaid, E.E.S., Yokoyama, N. and Igarashi, I., Evaluation of a Fluorescence-Based Method for Antibabesial Drug Screening, Antimicrob Agents Chemother. 58: 4713-7, August, (2014). (Impact factor 4.415).
¶ Equally contributed with the first author
* Corresponding author
Further Publications
1. Mohamed Abdo Rizk*, Shimaa Abd El-Salam El-Sayed, Sabry Elkhodery. Vitamin C impact on proinflammatory cytokines production in lambs with pneumonic pasteurellosis. Submitted to Research in Veterinary Science
2. Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Naoaki Yokoyama, and Ikuo Igarashi. Evaluation of the in vitro and in vivo inhibitory effect of thymoquinone on Babesia and Theileria parasites. Submitted to Parasite & Vector.
3. Mahmoud AbouLaila, Amer AbdEl-Aziz, Mohamed Abdo Rizk, Akram Salama, Shimaa El-Sayed, Naoaki Yokoyama, and Ikuo Igarashi. 2018. Evaluation of the in vitro and in vivo inhibitory effects of enrofloxacin on the growth of Babesia and Theileria parasites. Experimental Parasitology
4. Mahmoud AbouLaila, Amer AbdEl-Aziz, Mohamed Abdo Rizk, Davaasoren Batdorj, Shimaa El- Sayed, Naoaki Yokoyama, and Ikuo Igarashi. 2018. Evaluation of the in vitro and in vivo inhibitory effects of apigenin and gallic acid on the growth of Babesia and Theileria parasites. Veterinary Parasitology
5. Mahmoud AbouLaila, Amer AbdEl-Aziz, Mohamed Abdo Rizk, Shimaa El-Sayed, Akram Salama, Davaasoren Batdorj, Naoaki Yokoyama, and Ikuo Igarashi. 2018. Evaluation of the in vitro and in vivo inhibitory effects of quercetin on the growth of Babesia and Theileria parasites. Parasitology Research
6. Mahmoud AbouLaila, Amer AbdEl-Aziz, Mohamed Abdo Rizk, Shimaa El-Sayed, Naoaki Yokoyama, and Ikuo Igarashi. 2018. Evaluation of the in vitro and in vivo inhibitory effects of myrrh oil on the growth of Babesia and Theileria parasites
7. Shimaa Abd El-Salam El-Sayed, Mohamed Abdo Rizk, Azirwan Guswanto, Mohamad Alaa Terkawi, Naoaki Yokoyama, and Ikuo Igarashi. Spherical Body Protein 1 and Spherical Body Protein 4 Cocktail Antigen for Global Detection of Babesia bovis Infection in Cattle. Submitted to Ticks and Tick-borne Diseases
8. Shimaa Abd El-Salam El-Sayed, Mahmoud Rezk AbouLaila, Mohamed Abdo Rizk¶ , Ahmed O. ElKhatam, Mosaad Hilali , Azaa Abdel-Wahab, Naoaki Yokoyama, and Ikuo Igarashi. An epidemiological survey of Theileria equi parasite in horses, and donkeys in Egypt. Ready for submission to Tropical Animal Health and Production.
¶ Equally contributed with the first author
* Corresponding author

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